Peptide sequencing service provides a powerful and indispensable solution for elucidating peptide structures, identifying post-translational modifications (PTMs), and enabling advanced protein characterization. Rather than being a single analytical method, this service integrates multiple technologies, ranging from high-resolution mass spectrometry (MS) and nano-liquid chromatography (nano-LC) to deep learning-based de novo sequencing algorithms, offering researchers a comprehensive platform for sequence-level insights.
The evolution of peptide sequencing reflects decades of technological progress. Beginning with Edman degradation in the 1950s, which allowed stepwise N-terminal amino acid sequencing, the field experienced a paradigm shift in the 1980s with the introduction of tandem mass spectrometry (MS/MS). The advent of Orbitrap, Q-TOF, and nano-LC further enhanced detection sensitivity and sequence coverage, especially for low-abundance and modified peptides. Most recently, the integration of deep learning algorithms like DeepNovo has enabled accurate, database-independent peptide sequencing, even in complex or novel biological samples.
Leveraging these technological advancements, MtoZ Biolabs’ peptide sequencing service delivers an end-to-end, high-precision sequencing solution tailored for proteomics research, biopharmaceutical development, and diagnostic discovery. Our platform empowers researchers and industry partners to decode peptide sequences with confidence, accelerating innovation across life science and therapeutic domains.
Applications and Research Value of Peptide Sequencing Service
Peptide sequencing service plays a pivotal role in advancing proteomics and biomedical research by enabling accurate identification of peptide sequences and post-translational modifications (PTMs). This capability is essential for understanding protein function, uncovering molecular mechanisms, and supporting therapeutic innovation. With applications that span drug development, precision medicine, biomarker discovery, and biopharmaceutical quality control, peptide sequencing serves as a core analytical component in life sciences.
1. Drug Discovery and Antibody Engineering
Determining the amino acid sequences of peptides is critical for the development of peptide-based therapeutics and engineered antibodies. MtoZ Biolabs utilizes high-resolution mass spectrometry and deep learning-based de novo sequencing to support peptide screening, optimization, and modification analysis. This ensures accurate sequence annotation, PTM detection, and rational design of biologically active peptides or antibody fragments.
2. Functional Proteomics and Mechanism Elucidation
Peptides are central to deciphering protein structure and function. By combining enzymatic digestion strategies, high-efficiency enrichment, and multi-fragmentation MS techniques, our service allows in-depth analysis of signaling peptides, active domains, and regulatory motifs. Researchers can use these insights to map protein-protein interactions, post-translational signaling pathways, or disease-associated proteoforms.
3. Biomarker Identification and Early Diagnosis
In clinical samples such as plasma, urine, or tissue lysates, identifying disease-associated peptides provides valuable diagnostic and prognostic information. MtoZ Biolabs offers ultra-sensitive detection and advanced bioinformatics workflows to uncover potential peptide biomarkers, facilitating early disease detection and personalized therapeutic development.
4. Biopharmaceutical Quality Control and Regulatory Support
For therapeutic proteins and peptide drugs, sequencing enables monitoring of sequence fidelity, PTM consistency, and lot-to-lot uniformity. Our platform supports detailed molecular characterization aligned with regulatory expectations, ensuring the safety, efficacy, and stability of biopharmaceutical products throughout development and manufacturing.
Workflow for Peptide Sequencing Service
MtoZ Biolabs provides clients with a comprehensive, professional, and efficient peptide sequencing service, with strict quality and process control at every step to ensure accurate and reliable results.
1. Sample Submission and Pre-Evaluation
Clients are advised to submit:
- 10–25 μg for short peptides (<30 amino acids)
- ≥50 μg for longer peptides (>30 amino acids)
Samples should exhibit >95% purity, be free of salts and protease inhibitors, and be transported in low-protein-binding tubes under cold-chain conditions. Our technical team performs a thorough pre-assessment to customize the digestion and enrichment strategy for each sample type.
2. Enzymatic Digestion and Peptide Enrichment
To maximize sequence coverage, we employ multiple endoproteinases (e.g., trypsin, Lys-C, Glu-C, Asp-N) and combine with solid-phase extraction (SPE) or titanium dioxide (TiO₂) enrichment methods when PTM analysis is required.
3. LC-MS/MS Data Acquisition
Peptides are separated using nano-flow liquid chromatography and analyzed by tandem mass spectrometry (MS/MS) on high-resolution instruments such as Orbitrap Fusion Lumos or Q Exactive HF. Fragmentation methods including HCD, CID, or ETD are applied to maximize sequence coverage and facilitate post-translational modification (PTM) identification.
4. Sequence Analysis and Bioinformatics Interpretation
Mass spectra are analyzed using a combination of database search tools (e.g., Mascot, Sequest, PEAKS) and de novo sequencing algorithms. Our expert bioinformatics pipeline reconstructs peptide sequences, identifies PTMs, and generates annotated reports with confidence scores, sequence coverage maps, and biological context interpretation.
Why Choose MtoZ Biolabs for Peptide Sequencing Service?
MtoZ Biolabs delivers a comprehensive, high-precision peptide sequencing service that integrates cutting-edge mass spectrometry technology, intelligent data analysis, and expert-level bioinformatics support. Our service is designed to support the diverse needs of researchers and biopharmaceutical companies by providing detailed insights into peptide sequences, PTMs, and protein function.
1. Advanced Analytical Platforms and Technical Excellence
We employ state-of-the-art mass spectrometry systems, including Orbitrap Fusion Lumos and Q Exactive HF, combined with nano-LC automated sample injection technology. This combination enables ultra-high resolution and sensitivity, ensuring comprehensive detection of peptides, including low-abundance and modified peptides. Our multi-mode fragmentation approach, including HCD, ETD, and EThcD, provides high-confidence peptide sequencing and precise localization of modification sites.
2. Intelligent Algorithms Integrated with Deep Learning
To enhance peptide sequence accuracy and PTM detection, MtoZ Biolabs integrates multiple database search engines with advanced deep learning algorithms. This dual approach allows for accurate de novo peptide sequencing and optimized identification of challenging peptides, such as antibody fragments and cyclic peptides. Custom model refinement ensures the highest level of accuracy for complex samples.
3. Transparent and Single-Pricing Structure
MtoZ Biolabs offers a transparent pricing model with no hidden fees or unexpected costs. Clients are provided with a clear, upfront project quote based on sample complexity and analysis requirements. This ensures a predictable budget, allowing researchers to focus on advancing their work without concerns about unforeseen charges.
4. High-Quality Data and Interactive Visual Reports
Our AI-powered bioinformatics platform integrates experimental data to deliver detailed peptide sequence reports. These reports are presented in interactive formats, allowing clients to explore spectra and sequence matching in real time. Our platform enhances data usability by providing intuitive visualizations, empowering researchers to make informed scientific decisions.
5. Comprehensive Technical Support and Tailored Services
MtoZ Biolabs provides end-to-end technical support, including personalized consultation and real-time project tracking via our LIMS system. Whether handling complex samples or offering high-throughput sequencing, we tailor our service to meet specific research needs. Our team ensures seamless communication and expert guidance, from sample preparation to data interpretation, facilitating smooth progress from start to finish.
What Could Be Included in the Report?
Recognizing that detailed, traceable, and clear experimental reports are fundamental to advancing scientific research and industrial progress, MtoZ Biolabs ensures that its peptide sequencing service reports are not only comprehensive and detailed but also flexibly tailored to specific client requirements. These reports support in-depth understanding of sample data, validation of research findings, and the design of subsequent experiments.
1. Comprehensive Experimental Overview
Details the sample origin, preparation workflow, enzymatic digestion strategy, enrichment protocols, and analytical setup. This section outlines the experimental logic and execution process to ensure traceability and methodological clarity.
2. Reagents, Instruments, and Parameters
Lists the specific reagents and consumables used, including enzyme types, labeling kits (if any), and chromatographic media. Also includes mass spectrometer models (e.g., Orbitrap Fusion Lumos, Q Exactive), acquisition settings, and software tools employed for data processing.
3. Total Ion Chromatograms and QC Assessment (if applicable)
Provides representative chromatograms, signal-to-noise evaluations, peak resolution metrics, and overall instrument performance. This section reflects the quality and reliability of raw signal acquisition.
4. Data Processing and Quantitative Overview (if applicable)
Summarizes the data preprocessing steps, such as baseline correction, noise filtering, and peak alignment, along with any quantitative analysis performed. This ensures clarity on how raw data are transformed into interpretable information.
5. Bioinformatics Interpretation
Presents database matching or de novo sequencing results, with sequence confidence scores and MS/MS spectra annotation. When applicable, includes post-translational modification (PTM) localization, modification type classification, domain predictions, and related pathway associations.
6. Complete Raw Data Files (Optional)
Upon request, clients can receive a comprehensive set of raw data files, including original mass spectrometry data, database search outputs, DeepNovo-derived results, interactive spectra, and more, to fully support diverse research requirements.
FAQ
MtoZ Biolabs follows a customer-centric philosophy, offering concise and informative responses to frequently asked questions to ensure comprehensive support before and after utilizing our peptide sequencing service.
Q1. How do I choose the appropriate peptide sequencing method?
For peptides with known or partially known sequences, database-assisted identification provides rapid and accurate results. For unknown sequences, modified peptides, or structurally complex targets, de novo sequencing based on deep learning algorithms is recommended.
Q2. How is the reliability of peptide sequencing results ensured?
We ensure high-confidence results through advanced algorithmic processing, expert-level manual validation, and rigorous quality control. Final reports include confidence scores, spectral evidence, and access to raw data for transparency and reproducibility.
Q3. Can I submit low-quantity samples?
Yes. MtoZ Biolabs employs high-sensitivity instrumentation and optimized workflows to analyze low-abundance peptides. Please consult our technical team for recommendations on minimal input amounts and sample preparation strategies.
Q4. Does MtoZ Biolabs support high-throughput peptide sequencing?
Yes. We offer high-throughput capabilities supported by automated nano-LC systems and scalable data analysis pipelines. This enables efficient processing of large sample batches for applications such as antibody discovery, peptide library screening, and large-scale proteomic studies.
Join MtoZ Biolabs to Advance the Frontier of Peptide Sequencing
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.
In the dynamic fields of life science research, drug development, biomarker discovery, and quality control, precise and efficient peptide sequencing service is a vital driver of innovation and discovery. MtoZ Biolabs, leveraging world-leading high-resolution mass spectrometry platforms, deep learning-driven algorithms, rigorous quality control processes, and extensive technical expertise, delivers trusted solutions for research and industry partners. Choosing MtoZ Biolabs enhances precision in scientific exploration, accelerates project progress, and ensures the reliability of product development. More importantly, it marks a significant step toward unlocking the secrets of life and advancing human health. Let’s work together to embark on a new era of peptide sequencing!
For inquiries about experiencing MtoZ Biolabs’ peptide sequencing service, or to obtain customized peptide sequencing service by mass spectrometry and peptide sequence analysis solutions, please contact us at:
Name: Terry
Company: MtoZ Biolabs
Email: marketing@mtoz-biolabs.com
Phone: +1-857-362-9535
Address: 155 Federal Street, Suite 700, Boston, MA 02110, USA
Country: United States
Website: https://www.mtoz-biolabs.com/
Comments (0)