Quantitative proteomics service encompasses a comprehensive array of scientific solutions designed to accurately measure protein abundance and post-translational modifications within complex biological systems. Built upon the foundation of quantitative proteomics—a specialized field within life sciences—this service leverages advanced mass spectrometry (MS) technologies, including both label-free and labeling strategies, to achieve systematic and highly precise protein quantification. The discipline of quantitative proteomics emerged as a result of pioneering work by experts in analytical chemistry and life sciences, particularly from the late twentieth century onward. As MS instrumentation and chromatographic techniques evolved and innovative labeling methodologies were developed, the field progressed from simple protein identification to enabling large-scale, quantitative, and systems-level investigations.
These technological advancements have made quantitative proteomics service an indispensable tool for unraveling disease mechanisms, identifying drug targets, validating clinical biomarkers, and advancing the broader field of functional genomics. Recognizing the transformative impact of these innovations, MtoZ Biolabs has introduced a full spectrum of quantitative proteomics service offerings. By integrating world-class mass spectrometry platforms, stringent data quality controls, and expert bioinformatics, MtoZ Biolabs empowers researchers with state-of-the-art protein quantification analysis solutions, supporting scientific discovery at every stage.
Technical Approaches of Quantitative Proteomics
MtoZ Biolabs' quantitative proteomics service is primarily based on two core technical approaches: label-free quantification and label-based quantification. Each method is tailored to address specific project requirements and sample types.
1. Label-Free Quantification Methods
Label-free quantification enables the direct and relative measurement of protein levels without the need for chemical or metabolic labeling. Using advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS), this strategy extracts peptide intensity information directly from raw mass spectrometry data.
(1) LFQ (Label-Free Quantification): A widely used approach that estimates protein abundance by integrating peptide signal intensities (peak areas) or counting MS/MS spectra associated with each protein. LFQ is typically based on data-dependent acquisition (DDA) and is highly scalable and cost-effective, making it ideal for large-scale studies or samples where labeling is impractical.
(2) DIA-Based Label-Free Quantification: Data-independent acquisition (DIA) is an alternative data acquisition strategy that fragments all detectable peptides across predefined mass-to-charge (m/z) windows. When combined with label-free quantification algorithms, DIA enables comprehensive proteome coverage and high reproducibility, especially suitable for large cohort studies requiring robust comparability.
Supported by optimized sample processing workflows and validated analytical pipelines, MtoZ Biolabs’ label-free quantification strategies ensure sensitive, consistent, and reproducible results across a wide range of biological samples.
2. Label-Based Quantification Methods
Label-based quantification offers precise multiplexed analysis and high inter-sample comparability by incorporating stable isotopic or isobaric tags into proteins or peptides prior to LC-MS/MS.
(1) TMT/iTRAQ (Isobaric Labeling): These chemical labeling techniques use isobaric tags that react with peptide amines, enabling up to 10 samples to be analyzed in a single MS run. Quantification is achieved by measuring reporter ions generated during MS/MS. TMT and iTRAQ are ideal for high-throughput comparative analysis and offer fixed sample multiplexing with excellent data uniformity.
(2) SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture): A metabolic labeling method in which cells incorporate isotope-labeled amino acids into newly synthesized proteins. SILAC enables accurate and reproducible quantification with minimal variation, especially suited for dynamic proteomics and interaction studies in cell culture systems.
MtoZ Biolabs provides full support for label-based experiments, from optimized labeling protocols to downstream LC-MS/MS and quantitative bioinformatics interpretation.
3. Guidance for Method Selection
The choice of quantification strategy depends on specific study goals, sample availability, experimental scale, and budgetary considerations:
(1) LFQ (DDA-based) and DIA-based quantification are optimal for large-scale, cost-sensitive studies involving biofluids, tissues, or complex cohorts.
(2) TMT/iTRAQ are ideal for comparative analysis of medium-scale sample sets requiring high data consistency.
(3) SILAC is recommended for controlled cell-based studies emphasizing dynamic changes and precision quantification.
MtoZ Biolabs provides personalized consultation, ensuring every client benefits from the most suitable protein quantification analysis solutions to maximize research outcomes.
Applications of Quantitative Proteomics
MtoZ Biolabs' quantitative proteomics service empowers a broad spectrum of life science research by delivering stable and reliable protein quantification data. Additionally, our protein quantification analysis solutions offers a robust proteomics foundation for research in systems biology, environmental sciences, and clinical translational studies.
1. Disease Mechanism Research
Enables differential protein screening and pathway enrichment analyses, supporting the elucidation of molecular changes in health and disease.
2. Drug Target Validation
Measures protein expression changes before and after treatment, facilitating studies on drug mechanisms and therapeutic efficacy.
3. Tumor Subtype Classification
Utilizes quantitative protein expression profiles to classify tumor types, thereby informing precision medicine approaches.
4. Biomarker Screening and Validation
Provides comprehensive and targeted quantitative analyses to advance biomarker discovery and clinical validation.
5. Synthetic Biology and Metabolic Engineering
Supports the quantitative assessment of engineered biological pathways and microbial production strains.
From Sample to Insight: MtoZ Biolabs' End-to-End Workflow
The end-to-end workflow of MtoZ Biolabs' quantitative proteomics service ensures that every project is conducted with scientific rigor, precision, and full transparency.
1. Project Consultation and Experimental Design
Assess scientific objectives and project requirements. Provide expert guidance on selecting optimal quantification strategies and designing experiments with appropriate sample controls and replicates.
2. Sample Reception and Pre-Treatment
Perform thorough quality checks and standardization of all incoming samples. Conduct pre-processing steps, including lysis, enzymatic digestion (such as trypsinization), desalting, and labeling where necessary.
3. LC-MS/MS Mass Spectrometry Analysis
Employ advanced platforms (Orbitrap Exploris 480, QE HF-X, TIMS-TOF Pro) and tailor chromatography and MS acquisition parameters according to the chosen workflow (DDA, DIA, or MRM).
4. Data Quality Control and Quantitative Analysis
Process data using leading software tools (MaxQuant, Spectronaut, proprietary TMT/iTRAQ solutions). Establish multiple quality control checkpoints and apply robust statistical analyses to accurately identify significant protein changes. Correct for batch effects as required.
5. Bioinformatics Analysis and Visualization
Conduct comprehensive functional annotation, clustering, and advanced visualizations (including heatmaps, volcano plots, and pathway diagrams). Integrate artificial intelligence-powered analyses to accelerate and enhance data interpretation.
What Could Be Included in the Report?
Each quantitative proteomics service project at MtoZ Biolabs is delivered with a comprehensive, publication-ready report. The final content is tailored according to the project scope and specific client requirements, and may include the following sections:
1. Experimental Workflow
A detailed description of the overall experimental process, including sample receipt and preparation, protein extraction and digestion, optional labeling (e.g., TMT/iTRAQ/SILAC), chromatographic separation, and mass spectrometry acquisition. The full workflow ensures traceability and high reproducibility.
2. Materials, Instruments, and Methods
A complete list of reagents used (e.g., trypsin, labeling reagents), buffer compositions, LC-MS/MS run parameters, and instrument platforms. Standard operating procedures (SOPs) are provided for core steps.
3. TIC and Quality Control Assessment (project-dependent)
Visual and quantitative assessment of data quality, including total ion chromatograms (TIC), peptide length distribution, protein coverage, replicate reproducibility (e.g., R² values), and other key quality control metrics.
4. Data Processing and Quantitative Analysis (project-dependent)
A step-by-step overview of data analysis, covering protein and peptide identification strategies, data normalization (e.g., LFQ intensity scaling, correction factors), statistical analysis of differential expression, and software tools used (e.g., MaxQuant, Spectronaut, Perseus, R/Python scripts).
5. Bioinformatics Interpretation
Comprehensive biological analysis such as Gene Ontology (GO) annotation, pathway enrichment (KEGG/Reactome), subcellular localization, protein–protein interaction (PPI) network analysis, and clustering heatmaps, offering biological insight and intuitive visualizations of the results.
6. Raw Data and Supplementary Files
Full access to raw MS data files, processed result tables (e.g., differential protein lists, normalized intensity matrices), annotated graphs and visual outputs, along with documentation to support secondary analysis and seamless integration into scientific publications.
Why Choose MtoZ Biolabs' Quantitative Proteomics Service?
1. Advanced Analysis Platform
Our advanced mass spectrometry platform, including Orbitrap Exploris 480, QE HF-X, and TIMS-TOF Pro, supports all mainstream quantitative strategies (LFQ, DIA, TMT/iTRAQ, SILAC). This guarantees reliable, fast, and highly accurate analysis service with exceptional sensitivity and a broad dynamic range.
2. One-Time-Charge
Our pricing is transparent with a one-time-charge model, ensuring no hidden fees or additional costs. This allows you to clearly plan and manage your research budget without concerns.
3. High Data Quality
We ensure deep data coverage combined with strict data quality control throughout the entire project—from sample reception to final analysis. Interim quality reports and full sample traceability are provided, delivering consistent, reproducible, and statistically robust data results.
4. AI-Powered Bioinformatics
Our AI-powered bioinformatics platform integrates all proteomics data efficiently, providing clients with comprehensive data reports. This accelerates data interpretation and ensures the reliability and consistency of the results.
5. Extensive Project Experience
MtoZ Biolabs has participated in a variety of quantitative proteomics projects in collaboration with universities, research hospitals, and industry partners. Our team has experience in addressing diverse sample types and accommodating different analytical needs, including tailored quantification strategies and integration with other omics datasets.
6. One-Stop Service
From initial consultation and experimental design, through sample preparation and LC-MS/MS analysis, to final data interpretation and publication support, we provide comprehensive, end-to-end quantitative proteomics services. Our experts are always available to answer technical questions and provide ongoing guidance throughout your research project.
Advance Your Research with MtoZ Biolabs' Quantitative Proteomics Service
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.
Quantitative proteomics is a vital analytical approach in modern life science, driving insights in disease research, drug development, biomarker discovery, and synthetic biology. Leveraging advanced instrumentation and expert bioinformatics, MtoZ Biolabs provides comprehensive, flexible protein quantification analysis solutions tailored to diverse protein quantification needs.
To learn how our quantitative proteomics service can support your research goals, contact MtoZ Biolabs. We offer end-to-end assistance—from sample preparation to data interpretation—empowering confident, publication-ready results.
Contact us for a free consultation:
Name: Terry
Company: MtoZ Biolabs
Email: marketing@mtoz-biolabs.com
Phone: +1-857-362-9535
Address: 155 Federal Street, Suite 700, Boston, MA 02110, USA
Country: United States
Website: https://www.mtoz-biolabs.com/
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